Surface Plasmon Resonance Biosensor Research Articles

Design and Modelling of Surface Plasmon Resonance Biosensor Employing BaTiO3 and Graphene Nanostructure for Detection of SARS-CoV-2 Virus

Design and Modelling of Surface Plasmon Resonance Biosensor Employing BaTiO3 and Graphene Nanostructure for Detection of SARS-CoV-2 Virus

Ultrasensitive DNA Origami Plasmon Sensor for Accurate Detection in Circulating Tumor DNAs

AbstractEarly lung cancer screening by computed tomography is hampered by pulmonary nodules caused by massive COVID‐19 infections, necessitating an ultrasensitive approach for the early diagnosis of lung cancers at the single‐base level from circular tumor DNAs (ctDNAs). This study introduces an approach that merges DNA origami and DNA scissors technologies in a framework of surface plasmon resonance (SPR) biosensors. By combining the precision of DNA origami probes with the inherent single‐base resolution of DNA scissors, this method systematically addresses the limitations of conventional SPR techniques, resulting in enhanced detection accuracy. The synergistic interplay between DNA scissors and DNA origami enables the SPR biosensors to achieve unprecedented levels of sensitivity, precision, and practical utility. This efficacy allows the precise identification of mutations, demonstrated here by detection of the T790M mutation in the EGFR gene and the G12C mutation in the KRAS gene of non‐small cell lung cancer patients. With this technique, single‐base resolution as well as near zeptomolar‐level sensitivity is achieved. As a result, this discovery holds significant potential to advance the field of precision diagnostics.

Optimization of Cyanocobalamin (Vitamin B12) Sorption onto Mesoporous Superparamagnetic Iron Oxide Nanoparticles.

The techniques used to detect and quantify cyanocobalamin (vitamin B12) vary considerably in terms of detection sensitivity, from the most sensitive, based on radioisotopes and mass spectrometry (MS) with limits of detection (LOD) in fg mL-1, to fluorescence (FL) and surface plasmon resonance (SPR) biosensors with LOD values in the range of a few µg mL-1. For accurate quantification of an analyte present at trace levels in complex biological matrices, a selective separation and enrichment step is required to overcome matrix interferences and ensure sufficient detection sensitivity. In this study, iron oxide magnetic nanoparticles (IONPs) were used for the extraction and initial preconcentration of cyanocobalamin (vitamin B12). In the dependence of the magnetization on the H-field (hysteresis loop), no coercivity and remanence values were found at 300 K, indicating the superparamagnetic properties of the tested IONPs. Perfluorinated acids were used as amphiphilic agents to allow the sorption of cyanocobalamin onto the IONPs. FT-IR/ATR spectroscopy was used to confirm the sorption of cyanocobalamin on the IONPs. The influence of the addition of a homologous series of perfluorinated acids such as trifluoroacetic acid (TFAA), heptafluorobutyric acid (HFBA), and trichloroacetic acid (TCAA) to the extraction mixture was tested considering their type, mass, and time required for effective sorption. The adsorption kinetics and isotherm, described by the Freundlich and Langmuir equations, were analyzed. The maximum adsorption capacity (qm) exceeded 6 mg g-1 and was 8.9 mg g-1 and 7.7 mg g-1 for HFBA and TCAA, respectively, as the most efficient additives. After the desorption process using aqueous KH2PO4 solution, the sample was finally analyzed spectrophotometrically and chromatographically. The IONP-based method was successfully applied for the isolation of cyanocobalamin from human urine samples. The results showed that the developed approach is simple, cheap, accurate, and efficient for the determination of traces of cyanocobalamin in biological matrices.

Sensitive surface plasmon resonance biosensor by optimized carboxylate functionalized carbon nanotubes/chitosan for amlodipine detecting

The adoption of biophotonic sensing technologies holds significant promise for application in health care and biomedical industries in all aspects of human life. Then, this piece of writing employs the powerful effective medium theory and FDTD simulation to anticipate the most favorable state and plasmonic attributes of a magnificent nanocomposite, comprising carboxylate functionalized carbon nanotubes and chitosan (CS). Furthermore, it thoroughly explores the exhibited surface plasmon resonance behaviors of this composite versus the quantity of CS variation. Subsequently, enlightening simulations are conducted on the nanocomposite with a delicate layer and a modified golden structure integrating as a composite. The intricate simulations eventually unveil an optimal combination to pave the way for crafting an exceptional specific biosensor that far surpasses its counterpart as a mere Au thin layer in terms of excellence. The proposed biosensor demonstrated linear behavior across a wide range from 0.01 μM to 150 μM and achieved a detection limit of 10 nM, with a sensitivity of 134◦RIU−1.

Multi-channel prismatic localized surface plasmon resonance biosensor for real-time competitive assay multiple COVID-19 characteristic miRNAs

A multi-channel prismatic localized surface plasmon resonance (LSPR) biosensor was developed for quantitative and real-time detection of multiple COVID-19 characteristic miRNAs. The well-dispersed and dense gold nanoparticles (AuNPs) arrays for LSPR biosensing were fabricated through a nano-thickness diblock copolymer template (BCPT). Both theoretical and experimental analyses were conducted to investigate the effects of particle size, interparticle spacing, and surface coverage on LSPR sensing spectrum and intensity sensitivity of varied AuNPs sizes. A competitive assay strategy was proposed and used for non-amplification miRNA detection with a low limit detection of 3.41 nM, while a four-channel prismatic LSPR system enables parallel detection of multiple miRNAs. Furthermore, this sensing strategy can effectively and specifically identify target miRNA, distinguish mismatched miRNA and interfering miRNA, and exhibit low non-specific adsorption. This BCPT-based LSPR biosensor demonstrates the practicality and potential of a multi-channel, adaptable, and integrated prismatic sensor in medical testing and diagnostic applications.

Surface Plasmon Resonance Biosensor with High Sensitivity for Detecting SARS-CoV-2

Surface Plasmon Resonance Biosensor with High Sensitivity for Detecting SARS-CoV-2

Coffee Ring Effect Enhanced Surface Plasmon Resonance Imaging Biosensor via 2-λ Fitting Detection Method.

SPR biosensors have been extensively used for investigating protein-protein interactions. However, in conventional surface plasmon resonance (SPR) biosensors, detection is limited by the Brownian-motion-governed diffusion process of sample molecules in the sensor chip, which makes it challenging to detect biomolecule interactions at ultra-low concentrations. Here, we propose a highly sensitive SPR imaging biosensor which exploits the coffee ring effect (CRE) for in situ enrichment of molecules on the sensing surface. In addition, we designed a wavelength modulation system utilizing two LEDs to reduce the system cost and enhance the detection speed. Furthermore, a detection limit of 213 fM is achieved, which amounts to an approximately 365 times improvement compared to traditional SPR biosensors. With further development, we believe that this SPR imaging system with high sensitivity, less sample consumption, and faster detection speed can be readily applied to ultra-low-concentration molecular detection and interaction analysis.

A dual-target SPR screening system for simultaneous ligand discovery of SARS-CoV-2 spike protein and its receptor ACE2 from Chinese herbs

Traditional Chinese Medicine (TCM) is a supremely valuable resource for the development of drug discovery. Few methods are capable of hunting for potential molecule ligands from TCM towards more than one single protein target. In this study, a novel dual-target surface plasmon resonance (SPR) biosensor was developed to perform targeted compound screening of two key proteins involved in the cellular invasion process of the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2): the spike (S) protein receptor binding domain (RBD) and the angiotensin-converting enzyme 2 (ACE2). The screening and identification of active compounds from six Chinese herbs were conducted taking into consideration the multi-component and multi-target nature of Traditional Chinese Medicine (TCM). Puerarin from Radix Puerariae Lobatae was discovered to exhibit specific binding affinity to both S protein RBD and ACE2. The results highlight the efficiency of the dual-target SPR system in drug screening and provide a novel approach for exploring the targeted mechanisms of active components from Chinese herbs for disease treatment.

Smartphone-based selective and sensitive detection of vitamin B1 in synthetic urine using U-bend SPR optical fiber probe

This paper introduces an innovative optical fiber surface plasmon resonance (SPR) biosensor designed for smartphone assistance, aiming at detecting vitamin B1 in synthetic urine. The key novelty lies in the amalgamation of silver nanocoated cysteine with a gold-plated optical fiber probe, fostering efficient detection through the robust electrostatic interaction between negatively charged Cys-capped AgNPs and positively charged vitamin B1 molecules. Notably, this biosensor leverages the smartphone's digital camera capabilities to discern varying color intensities. A self-developed application ensures real-time monitoring of green and red channel intensities in the SPR signal, thereby mitigating the risk of false detections. Moreover, the integration of the fabricated optical fiber probe with the smartphone is achieved through a 3D-printed casing, endowing the biosensor with the advantages of portability, handheld operation, and cost-effectiveness. The proposed U-shaped optical fiber SPR biosensor, coupled with smartphone technology, demonstrates the ability to detect vitamin B1 within the concentration range of 2–10 μM, yielding a commendable R2 value of 0.9973. Experimental findings affirm that this novel biosensor, built on the U-shaped SPR optical fiber architecture and empowered by smartphones, is an economical, uncomplicated, and portable device adept at accurately and efficiently detecting vitamin B1 across a broad concentration spectrum. Its potential applications extend to the area of low-cost biochemical component identification.

A new surface plasmon resonance-based immunoassay for rapid and sensitive quantification of D-dimer in human plasma for thrombus screening

D-dimer is a protein fragment generated during the fibrin breakdown by plasmin, and it serves as a mature biomarker for diagnosing thrombotic disorders. A novel immunoassay method based on surface plasmon resonance (SPR) has been developed, validated, and successfully applied for the quantification of D-dimer in human plasma with high sensitivity and rapidity. In this methodological study, we investigated the activity and stability of the SPR biosensor, sample pre-processing, washing conditions, intra-day and inter-day precision and accuracy and detection parameters, including a limit of detection of 8.3 ng/mL, a detection range spanning from 31.25 to 4000 ng/mL, and a detection time of 20 min. We compared D-dimer plasma concentration determination results using SPR with a classical latex-enhanced immunoturbidimetric immunoassay in 29 healthy individuals and thrombotic patients, and both methods exhibited consistency. Furthermore, we propose a hypothesis about the relationship between the concentration of D-dimer and its molecular weight. With an increase in the D-dimer concentration in plasma, the D-dimer approaches its simplest form (190 kDa).

PCF Based Four-Channel SPR Biosensor With Wide Sensing Range.

In this article, we have demonstrated a highly sensitive four-channel photonic crystal fiber (PCF) based surface plasmon resonance (SPR) biosensor which can detect four different analytes simultaneously. To ease practical implementation, four analyte sensing layers and plasmonic materials such as gold (Au) and gold (Au) with Tantalum Pentoxide (Ta2O5) are placed on the exterior of the four arms of the square shaped structure. The sensor's structure consists of only nine circular air holes, making it simple and easy to fabricate using currently available technologies. Finite element method (FEM) based numerical analysis is used to evaluate the sensing performance of the proposed sensor. With optimum structure parameters, the sensor achieves maximum wavelength sensitivity of 11000, 25000, 11000 and 25000 nm/RIU for Channel-1, Channel-2, Channel-3, and Channel-4 respectively. It shows maximum amplitude sensitivity of 803.732, 709.171, 803.827, 709.146 RIU -1 for Channel 1, 2, 3, and 4 respectively. It also shows maximum FOM of 232.55, 352.36, 231.57, 352.36 RIU -1 in Ch-1, Ch-2, Ch-3 and Ch-4 respectively. Moreover, the proposed sensor shows a wide range of refractive index sensing capability from 1.30 to 1.41. Due to multi-analyte detection capability, large sensing range, and excellent sensitivity the proposed sensor unfolds unrivalled capacity of detecting chemicals, carcinogenic agents, biomolecules, and other analytes.

Label-free biosensor on chip (BoC) based on localized surface plasmon resonance for monitoring temperature and blood hemoglobin concentration

On-chip localized surface plasmon resonance (LSPR) biosensor on chip (BoC) is a type of label-free biosensor that utilizes the plasmonic resonances of metal nanostructures to detect changes in the refractive index of the local environment. This results in changes in the intensity and wavelength of the surface plasmon resonance, which can be used to quantify the presence and concentration of biomolecules such as proteins, nucleic acids, and cells. In this paper, we propose a novel on-chip device with a microfluidic channel that contains the biological fluid under test. We have obtained sharp resonance peaks in the wavelength range between 700–800 nm with a sensitivity of 509 nm R−1IU−1 which is good compared to other on-chip devices. The main advantage of our design is the less complex manufacturing process compared to other BoCs. Our design consists of a central cavity that is surrounded by silver and consists of rectangular pillar-shaped silver particles placed in the cavity. Two very promising applications of this device are label-free temperature sensing and blood hemoglobin (Hb) concentration sensing with a resolution of 0.222 nm/°C for temperature and 1. 34 nm/(g/dL) for Hb. However, it can be used for any kind of sensing application that involves refractive index changes as the sensing platform.

Magnesium fluoride and black phosphorus mediated long-range surface plasmon resonance biosensor for enhanced sensing of SARS-CoV-2 virus

Magnesium fluoride and black phosphorus mediated long-range surface plasmon resonance biosensor for enhanced sensing of SARS-CoV-2 virus

Ferroelectric Bi2O2Te-Based Plasmonic Biosensor for Ultrasensitive Biomolecular Detection.

Ultrasensitive detection of biomarkers, particularly proteins, and microRNA, is critical for disease early diagnosis. Although surface plasmon resonance biosensors offer label-free, real-time detection, it is challenging to detect biomolecules at low concentrations that only induce a minor mass or refractive index change on the analyte molecules. Here an ultrasensitive plasmonic biosensor strategy is reported by utilizing the ferroelectric properties of Bi2O2Te as a sensitive-layer material. The polarization alteration of ferroelectric Bi2O2Te produces a significant plasmonic biosensing response, enabling the detection of charged biomolecules even at ultralow concentrations. An extraordinary ultralow detection limit of 1fm is achieved for protein molecules and an unprecedented 0.1fm for miRNA molecules, demonstrating exceptional specificity. The finding opens a promising avenue for the integration of 2D ferroelectric materials into plasmonic biosensors, with potential applications spanning a wide range.

SPR and Double Resonance LPG Biosensors for Helicobacter pylori BabA Antigen Detection.

Given the medical and social significance of Helicobacter pylori infection, timely and reliable diagnosis of the disease is required. The traditional invasive and non-invasive conventional diagnostic techniques have several limitations. Recently, opportunities for new diagnostic methods have appeared based on the recent advance in the study of H. pylori outer membrane proteins and their identified receptors. In the present study we assess the way in which outer membrane protein-cell receptor reactions are applicable in establishing a reliable diagnosis. Herein, as well as in other previous studies of ours, we explore the reliability of the binding reaction between the best characterized H. pylori adhesin BabA and its receptor, the blood antigen Leb. For the purpose we developed surface plasmon resonance (SPR) and double resonance long period grating (DR LPG) biosensors based on the BabA-Leb binding reaction for diagnosing H. pylori infection. In SPR detection, the sensitivity was estimated at 3000 CFU/mL-a much higher sensitivity than that of the RUT test. The DR LPG biosensor proved to be superior in terms of accuracy and sensitivity-concentrations as low as 102 CFU/mL were detected.

Tuning optical properties of Au thin film using electric field for surface plasmon resonance biosensor application

The effect of electric field application on the optical properties of gold (Au) thin film for spectroscopic ellipsometry-based surface plasmon resonance (SPR) biosensor applications has been successfully studied. The optical properties, such as the complex refractive index, complex dielectric constant, real optical conductivity, and absorption coefficient, were determined by fitting data from the ψ and Δ functions obtained through measurements using a homemade rotating analyzer ellipsometer. The applied electric fields of 0.8 V/cm, 1.6 V/cm, 2.4 V/cm, and 3.2 V/cm were induced by applying voltages of 1 V, 2 V, 3 V, and 4 V for ellipsometer tests, and 1.6 V/cm to 3.2 V/cm for the SPR test. The Au thin film was deposited onto a glass slide using an evaporator for ellipsometer testing and a BK7 half-cylinder prism was employed for SPR testing. The experimental results show that the real part of the refractive index (n) increases in the spectral range from 1.5 eV to 2.2 eV and from 3 eV to 5 eV and tends to be constant in the range from 2.3 eV to 2.8 eV. Conversely, the imaginary part of the refractive index (k) decreases from 2.8 eV to 3.6 eV and tends to remain consistent from 3.9 eV to 5.0 eV. The dielectric constant (ε1) consistently shows a significant increase within the spectral range of 3 eV–5.0 eV, with a tendency to decrease between 2.1 eV and 2.4 eV. Meanwhile, the imaginary part (ε2) demonstrates an increase in the spectral range of 1.5 eV–2.4 eV and 3.3 eV–5.0 eV while maintaining relatively stable values in the energy spectral range of 2.6 eV–3.0 eV. Moving to the real part of optical conductivity (σ1), it increases within the spectral range from 1.5 eV to 2.4 eV and from 3.5 eV to 5 eV, with relatively constant values between 2.6 eV and 3.1 eV. The absorption coefficient (α) exhibits a decrease in the spectrum between 2.8 eV and 3.6 eV, but the values remain stable within the range of 3.9 eV–5.0 eV. The α slightly decreases in the energy range of 1.8 eV–2.6 eV. The outcomes obtained from these experiments are directly linked to the SPR results, revealing a noticeable shift in the SPR angle (θSPR) of 45.51°, 45.61°, and 45.73° and an increase in the minimum reflectance (Rmin) of 0.05, 0.09, and 0.13 for electric field strengths of 0 V/cm, 1.6 V/cm, and 3.2 V/cm, respectively. These results imply that the application of an external electric field to the surface of the Au thin film disrupts the matching condition between the evanescent wave vector and the surface plasmon wave vector, thereby altering the reflected light under resonance conditions indicated by an increase in the minimum reflectance and SPR angle. This development holds significant promise to further enhance the sensitivity of electro-optic modulation-based biosensors.

High-throughput BCRP inhibitors screening system based on styrene maleic acid polymer membrane protein stabilization strategy and surface plasmon resonance biosensor

Multidrug resistance (MDR) is a dominant challenge in cancer chemotherapy failure. The over-expression of breast cancer resistance protein (BCRP) in tumorous cells, along with its extensive substrate profile, is a leading cause of tumor MDR. Herein, on the basis of styrene maleic acid (SMA) polymer membrane protein stabilization strategy and surface plasmon resonance (SPR) biosensor, a novel high-throughput screening (HTS) system for BCRP inhibitors has been established. Firstly, LLC-PK1 and LLC-PK1/BCRP cell membranes were co-incubated with SMA polymers to construct SMA lipid particles (SMALPs). PK1-SMALPs were thus immobilized in channel 1 of the L1 chip as the reference channel, and BCRP-SMALPs were immobilized in channel 2 as the detection channel to establish the BCRP-SMALPs-SPR screening system. The methodological investigation demonstrated that the screening system was highly specific and stable. Three active compounds were screened out from 26 natural products and their affinity constants with BCRP were determined. The KD of xanthotoxin, bergapten, and naringenin were 5.14 μM, 4.57 μM, and 3.72 μM, respectively. The in vitro cell verification experiments demonstrated that xanthotoxin, bergapten, and naringenin all significantly increased the sensitivity of LLC-PK1/BCRP cells to mitoxantrone with possessing reversal BCRP-mediated MDR activity. Collectively, the developed BCRP-SMALPs-SPR screening system in this study has the advantages of rapidity, efficiency, and specificity, providing a novel strategy for the in-depth screening of BCRP inhibitors with less side effects and higher efficacy.

An investigation of the influence of the graphene layers number on the performance of surface plasmon resonance biosensors with various thicknesses of silver

An investigation of the influence of the graphene layers number on the performance of surface plasmon resonance biosensors with various thicknesses of silver

High sensitive optical fiber SPR sensor for label-free detection of Staphylococcus aureus

Optical fiber bacterial sensors have attracted attention in many fields due to their unique advantages. However, it is still difficult to promote its practicality which is mainly subject to their low sensitivity, inferior stability, and reliability. This work reported here an optical fiber surface plasmon resonance (SPR) biosensor enhanced by gold nanoparticles (AuNPs)/Au film coupling for label-free detection the Staphylococcus aureus (S. aureus), while both the gold substrates were successfully biofunctionalized with goat anti-human immunoglobulin G (IgG) as the probe to capture and detect the bacteria. In the experiment, the refractive index（RI） sensitivity of the Multimode-Singlemode-Multimode optical fiber (MSMOF) coated with Au film was improved by 19 % after the AuNPs were homogeneously decorated on the Au film surface. To detect S. aureus by the sensor, the SPR wavelength variation in the transmission spectrum was recorded, and its limit of detection (LOD) was demonstrated experimentally to be about 50 CFU/mL and was obtained theoretically to be 1.18 CFU/mL during its concentration range of 50–108 CFU/mL. Also, it shows great linearity in the range of 102-108 CFU/mL and the sensor exhibits excellent specificity and stability. This kind of SPR-enhanced optical fiber biosensor is fully promising for its applications in biomedicine, food safety, pharmaceuticals, etc.

Analysis of Tapered Fibre Optic Surface Plasmon Resonance Bio-Sensor Chip with highly Perturbed Taper Profiles.

A numerical model based on the Transfer matrix method (TMM) is proposed for the first time to study the gold coated tapered fibre optic surface plasmon resonance (SPR) with eight different types of taper profiles namely linear, exponential-linear, Gaussian, quadratic, sinusoidal, error function type and highly perturbed taper profile so-called chirp type of profile. The performance in terms of sensitivity, full width at half maximum (FWHM), detection accuracy (D.A.), amplitude dip, and half power points are estimated with respect to tapering ratio and choices of taper profile. It is found that sensitivity increased almost linearly with the taper ratio of each taper choice for the account of the reduction of detection accuracy. It has been found that sensitivity is highest for the case of chirp taper profile and lowest for the case of quadratic taper profile at low taper ratio. In this study, the aqueous solution is considered for sensor development which is adulterated by biomolecules species like DNA, blood samples, etc.